ABSTRACT
Clavicle fracture, a very rare delayed complication following radical neck dissection of oral carcinoma, is normally ignored by oral and maxillofacial surgeons. We report and analyze a male patient with clavicle fracture after primary extended excision and bilateral radical neck dissection. This case was misdiagnosed as cervical metastasis.
Subject(s)
Humans , Male , Clavicle , Fractures, Bone , Mouth Floor , Mouth Neoplasms , Neck DissectionABSTRACT
An effective method was developed for determination of pyraclostrobin and BF 500-3 residues in pollen and honey of litchi by QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry. The samples were extracted by acetonitrile and cleaned by primary secondary amine(PSA) and C18. In the positive ion mode and multi reaction monitoring mode,the analytes were quantified by the matrix matching standard solutions, and the pretreatment and mass spectrometry conditions were evaluated. The matrix matched standard solutions of pyraclostrobin and its metabolite BF 500-3 showed good linearities in the concentration range of 1-100 μg/L, with the correlation coefficients (R2) of 0.991-0.999. The average recoveries were 87.0%-97.8% with relative standard deviations of 1.3%-3.7%. The limit of detection (LOD) and the limit of quantification(LOQ) were 0.08-0.20 μg/kg and 0.20-0.50 μg/kg,respectively. The method was easy, quick and highly sensitive, and was suitable for quick determination of pyraclotrobin and its metabolites in pollen and honey of litchi.
ABSTRACT
<p><b>OBJECTIVE</b>To study the association between single nucleotide polymorphisms(SNP) in toll-like receptors (TLR) 2 and 5 genes and the susceptibility to neonatal sepsis.</p><p><b>METHODS</b>One hundred and fourteen newborn infants who were diagnosed with clinical sepsis (case group) between May 2011 and January 2014 and 172 newborn infants without infection(control group) were enrolled in this study. The polymorphisms of TLR2 (rs5743708 and rs3804099) and TLR5 (rs5744105) were analyzed using a SNaPshot multiplex reaction to compare the genotypic and allelic frequencies between two groups. The relationship between TLR genotypes and susceptibility to sepsis was analyzed by logistic regression models.</p><p><b>RESULTS</b>Significant differences in genotypic frequencies of TLR2 rs3804099 (C/T) and TLR5 rs5744105 (C/G) were found between the two groups (P<0.05), but there was no significant difference in allelic frequencies of all the SNPs above between the two groups (P>0.05). The genotype on TLR2 rs5743708 was GG and no mutation was found in both groups. In regression models, birth weight (OR=3.065; P<0.05) and gestational age (OR=3.301; P<0.05) were closely associated with neonatal sepsis. Sex (OR=1.107, P>0.05), polymorphisms in rs3804099 (OR=0.876; P>0.05) and polymorphisms in rs5744105 (OR=0.820; P>0.05) genes were not risk factors for neonatal sepsis.</p><p><b>CONCLUSIONS</b>TLR2 and 5 polymorphisms (rs5743708, rs3804099 and rs5744105) may not serve as the susceptible gene for sepsis in newborn infants.</p>
Subject(s)
Female , Humans , Infant, Newborn , Male , Genetic Predisposition to Disease , Logistic Models , Polymorphism, Single Nucleotide , Sepsis , Genetics , Toll-Like Receptor 2 , Genetics , Toll-Like Receptor 5 , GeneticsABSTRACT
<p><b>OBJECTIVE</b>Study on the diagnostic accuracy of fine needle aspiration biopsy of cervical lymph nodes and to discuss the reasons of making a misdiagnosis.</p><p><b>METHODS</b>Five hundred and eighty cases of cervical lymph node fine needle aspiration biopsy were reviewed retrospectively. Among them, histologic findings were available in 161 cases. The cytologic and histologic diagnoses were compared.</p><p><b>RESULTS</b>There were altogether 226 cases of reactive lymphoid hyperplasia, 202 cases of specific inflammation, 45 cases of malignant lymphoma and 107 cases of metastatic carcinoma. The concordance rate between the cytologic and corresponding histologic diagnoses was 94.4%. The primary foci of most cases with metastatic carcinoma could be delineated by reviewing the clinical and pathologic finding. Inadequate cellularity was the main reason of making misdiagnosis and useful diagnostic clues might be obtained by careful study of the clinical findings.</p><p><b>CONCLUSIONS</b>Fine needle aspiration biopsy of cervical lymph node carries a high diagnostic accuracy. It provides important clues in guiding subsequent clinical management. However, for detailed subtyping of certain disease entities such as malignant lymphoma, surgical biopsy for histologic and immunohistochemical studies are required.</p>